Detection of Naegleria sp. in a Thermal, Acidic Stream in Yellowstone National Park

KATHY B. SHEEHAN; MICHAEL J. FERRIS; JOAN M. HENSON

doi:10.1111/j.1550-7408.2003.tb00132.x

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1 July 2003 Detection of Naegleria sp. in a Thermal, Acidic Stream in Yellowstone National Park

KATHY B. SHEEHAN, MICHAEL J. FERRIS, JOAN M. HENSON

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The J. of Eukaryotic Microbiology, 50(4):263-265 (2003). https://doi.org/10.1111/j.1550-7408.2003.tb00132.x

Abstract

An initial survey of sequences of PCR-amplified portions of the 18S rRNA genes from a community DNA clone library, prepared from an algal mat in a thermal, acidic stream in Yellowstone National Park, WY, USA, revealed among other sequences, several that matched Vahlkampfia. This finding prompted further investigation using primers specific for Naegleria. Sequences from a subsequent DNA clone library, prepared from the 5.8S rRNA gene and the adjacent internal transcribed spacer (ITS) regions of the rRNA, closely matched Naegleria and formed an independent lineage within a clade containing Naegleria sturti and Naegleria niuginiensis. The sequences may represent a new Naegleria species.

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KATHY B. SHEEHAN, MICHAEL J. FERRIS, and JOAN M. HENSON "Detection of Naegleria sp. in a Thermal, Acidic Stream in Yellowstone National Park," The Journal of Eukaryotic Microbiology 50(4), 263-265, (1 July 2003). https://doi.org/10.1111/j.1550-7408.2003.tb00132.x

Received: 21 December 2001; Accepted: 2 April 2003; Published: 1 July 2003

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