Mist-netting and acoustic monitoring was used to determine occurrence, activity, and use of habitats by bats at Montezuma Castle (i.e., Montezuma Castle Unit and Montezuma Well Unit), Tonto, and Tuzigoot national monuments, Arizona. All calls were visualized by generating sonograms and identified to species except for Myotis, because of similarity between calls within this genus. We used discriminate analysis to identify calls of Myotis to species. Riparian and marsh vegetational associations had the highest overall activity, except at Montezuma Castle Unit where desert, riparian, and transition vegetational associations had similar overall activity. Use of acoustic monitoring and mist-netting enabled us to detect one to eight more species, depending on site, than if only one technique had been used.