A study was conducted at the Louisiana State University Agricultural Center's H. Rouse Caffey Rice Research Station in 2017 and 2018 to evaluate the interaction between a prepackage mixture of clomazone plus pendimethalin applied at 0, 760, 1,145, or 1,540 g ai ha^–1 mixed with propanil at 0, 1,120, 2,240, or 4,485 g ai ha^–1. A synergistic response occurred when barnyardgrass was treated with all rates of clomazone plus pendimethalin mixed with either rate of propanil evaluated at 56 d after treatment (DAT). Unlike barnyardgrass, an antagonistic response occurred in yellow nutsedge used as a control when treated with 760 and 1,540 g ha^–1 of clomazone plus pendimethalin mixed with 1,120 or 22,40 g ha^–1 of propanil at 28 DAT; however, 1,145 g ha^–1 of clomazone plus pendimethalin mixed with 4,485 g ha^–1 of propanil resulted in a neutral interaction. At 28 DAT, rice flatsedge treated with all herbicide mixtures resulted in neutral interactions. The synergism of clomazone plus pendimethalin applied at 1,540 g ha^–1 mixed with propanil applied at 2,240 or 4,485 g ha^–1 to control barnyardgrass resulted in an increased rough rice yield compared with 760 or 1,145 g ha^–1 of clomazone plus pendimethalin mixed with propanil applied at 1,120 or 2,240 g ha^–1. These results indicate that if barnyardgrass and rice flatsedge are present in a rice field the prepackage mixture of clomazone plus pendimethalin mixed with propanil can be an option for growers. However, if yellow nutsedge infest the area other herbicides may be needed.

Nomenclature: clomazone; pendimethalin; propanil; barnyardgrass, Echinochloa crus-galli (L.) Beauv.; rice flatsedge, Cyperus iria (L.); yellow nutsedge, Cyperus esculentus (L.); rice, Oryza sativa (L.)

The application of paraquat mixtures with residual herbicides before planting rice is a common treatment in Mississippi, and rice in proximity is susceptible to off-target movement of these applications. Four concurrent studies were conducted in Stoneville, MS, to characterize rice performance following exposure to a sublethal rate of paraquat, metribuzin, fomesafen, and cloransulam-methyl at different application timings. Herbicides were applied to rice at the growth stages of spiking to one-leaf (VEPOST), two- to three-leaf (EPOST), three- to four-leaf (MPOST), 7 d postflood (PFLD), and panicle differentiation (PD). Regardless of application timing, rice injury following exposure to paraquat was ≥45%. Delays in maturity were increased by 0.3 d d^–1 following paraquat from emergence through PD. Dry weight, rough rice yield, panicle density, and germination were reduced by 18.7 g, 131.5 kg ha^–1, 5.6 m^–2, and 0.3%, respectively, per day from application of paraquat at emergence through PD. By 28 d after treatment (DAT), metribuzin injured rice 3% to 6%, and that injury did not translate into a yield reduction. Regardless of application timing, rice injury following fomesafen application ranged from 2% to 5% 28 DAT. Rice exposed to cloransulam-methyl EPOST exhibited the greatest root and foliar injury 21 DAT and 28 DAT, respectively. Additionally, when rice was exposed to cloransulam-methyl EPOST, yield was reduced to 6,540 kg ha^–1 compared with a yield of 7,850 kg ha^–1 from nontreated rice. Rice yield was negatively affected after paraquat was applied any time after rice emergence. However, applications of paraquat to rice at early reproductive growth stages reduced rough rice yield and seed germination the greatest. Application timing is crucial in determining severity of rice injury. Early-season injury to rice following paraquat application had less effect on yield compared with injury at later stages. Additionally, fields devoted to seed rice production are at risk for reduced seed germination if they are exposed to paraquat during early reproductive growth stages.

Nomenclature: Cloransulam-methyl; fomesafen; paraquat; metribuzin; rice; Oryza sativa L.

A field study was conducted in 2017 and 2018 at the Louisiana State University Agricultural Center H. Rouse Caffey Rice Research Station near Crowley, LA, to evaluate the impact of reduced rates of halosulfuron on quizalofop activity in Louisiana rice production. Halosulfuron and a prepackaged mixture of halosulfuron plus thifensulfuron were evaluated at 0, 17, 35, or 53 g ai ha^–1 and 34 or 53 g ai ha^–1, respectively, in a mixture with quizalofop at 120 g ai ha^–1. Control of barnyardgrass, red rice, and two non-acetyl-CoA carboxylase resistant rice lines, CL-111 and CLXL-745, were recorded at 14 and 28 d after treatment (DAT). The red rice, CL-111, and CLXL-745 represented a weedy rice population. Across all species evaluated at 14 DAT, all mixtures containing halosulfuron and halosulfuron plus thifensulfuron resulted in antagonism with an observed control of 79% to 90%, compared with an expected control of 96% to 99%. At 28 DAT, all mixtures containing halosulfuron resulted in neutral interactions for barnyardgrass control. Quizalofop mixed with halosulfuron plus thifensulfuron at the lower rate of 34 g ha^–1 was able to overcome the antagonism compared with the higher rate of 53 g ha^–1 for barnyardgrass control at 28 DAT. Both the high and the low rate of halosulfuron plus thifensulfuron resulted in antagonistic interaction for red rice, CL-111, and CLXL-745 control at 28 DAT. This research suggests that mixing quizalofop with halosulfuron plus thifensulfuron should be avoided, especially at the higher rate of 53 g ha^–1.

Nomenclature: Halosulfuron; halosulfuron plus thifensulfuron; barnyardgrass; Echinochloa crus-galli (L.) P. Beauv.; red rice; Oryza sativa L.; rice; Oryza sativa L.

In Bangladesh, weeds in transplanted rice are largely controlled by labor-intensive and costly manual weeding, resulting in inadequate and untimely weed control. Labor scarcity coupled with intensive rice production has triggered increased use of herbicides. These factors warrant a cost-effective and strategic integrated weed management (IWM) approach. On-farm trials with transplanted rice were conducted during monsoon (‘Aman’) season in 2016 and 2017 and winter (‘Boro’) season in 2016 to 2017 in agroecological zones 11 and 12 with ten treatments—seven herbicide-based IWM options, one mechanical weed control-based option, and two checks (farmers’ current weed control practice and weed-free)—to assess effects on weed control, grain yield, labor use, and profitability. Compared to farmers’ practice, herbicide-based IWM options with mefenacet + bensulfuron-methyl as preemergence followed by (fb) either bispyribac-sodium or penoxsulam as postemergence fb one hand-weeding were the most profitable alternatives, with reductions in labor requirement by 11 to 25 person-days ha^–1 and in total weed control cost by US$44 to 94 ha^–1, resulting in net returns increases by US$54 to 77 ha^–1 without compromising on grain yield. In contrast, IWM options with bispyrbac-sodium or penoxsulam as postemergence application fb one hand-weeding reduced yields by 12% to 13% and profits by US$71 to 190 ha^–1. The nonchemical option with mechanical weeding fb one hand-weeding performed similarly to farmers’ practice on yield and profitability. We suggest additional research to develop feasible herbicide-free approaches to weed management in transplanted rice that can offer competitive advantages to current practices.

Nomenclature: Bispyribac-sodium; mefenacet + bensulfuron-methyl; penoxsulam; rice, Oryza sativa L.

Smallflower umbrella sedge is one of the most problematic weeds in direct-seeded rice in India. Bispyribac-sodium (acetolactate synthase [ALS]-inhibiting herbicide) is commonly used in rice, but growers have recently reported lack of smallflower umbrella sedge control with this herbicide. An extensive survey was carried out in two rice-growing states, Chhattisgarh and Kerala, where 53 putative bispyribac-sodium-resistant (BR) biotypes were collected. Studies were conducted to confirm resistance to bispyribac-sodium and to test the efficacy of the newly developed synthetic auxin herbicide florpyrauxifen-benzyl on putative BR biotypes. A whole-plant bioassay revealed that bispyribac-sodium is no longer effective. Of 53 putative BR biotypes, 17 biotypes survived the recommended label rate of 25 g ai ha^–1. The effective bispyribac-sodium rate required to control 50% of the plants in most of the BR biotypes (ED₅₀) ranged from 19 to 96 g ha^–1, whereas it was 10 g ha^–1 in a susceptible biotype. In two highly resistant biotypes, the ED₅₀ was beyond the maximum tested rate, 200 g ha^–1. This suggests 2- to >20-fold resistance in BR biotypes. An ALS enzyme activity assay suggests an altered target site as mechanism of resistance to bispyribac-sodium. This study confirms the first case of evolved resistance to bispyribac-sodium in smallflower umbrella sedge in India. However, the newly developed synthetic auxin florpyrauxifen-benzyl effectively controlled all BR biotypes at the field use rate of 31.25 g ai ha^–1.

Nomenclature: Bispyribac-sodium; florpyrauxifen-benzyl; smallflower umbrella sedge, Cyperus difformis L.; rice, Oryza sativa L.

Introduction and rapid adoption of dicamba-resistant (DR) soybean led to an increase of post-emergent applications of dicamba. This resulted in a widespread increase in nontarget dicamba injury to non-DR soybean in 2017. Field studies were conducted in Manhattan, KS, in 2018 and 2019 and in Ottawa, KS, in 2019 to investigate the injury and yield response of soybean varieties with varying herbicide-resistance traits and maturity groups when exposed to dicamba. Four varieties were tested: ‘Credenz 3841LL’ (glufosinate resistant), ‘Credenz 4748LL’ (glufosinate resistant), ‘Asgrow AG4135RR2Y’ (glyphosate resistant), and ‘Stine 40BA02’ (glyphosate and isoxaflutole resistant), abbreviated as CR3841, CR4748, AG4135, and ST40B, respectively. Soybeans were treated with 5.6 g ae ha^–1 of dicamba at V3 and R1 stages. Percent soybean injury, soybean height, soybean yield and yield components, and injury to offspring were evaluated. Four weeks after treatment (WAT) at V3, the greatest injury was observed in AG4135 and ST40B. Dicamba application at R1 resulted in the greatest injury to ST40B both 4 WAT and at senescence. Minimal injury was observed in all varieties treated at V3 at senescence and yield loss was 5% or less. Dicamba application at R1 resulted in 19 to 34% yield loss, with the least yield loss in CR4748, and the greatest in ST40B. Varieties with greater injury at senescence generally yielded less than other varieties.

Nomenclature: dicamba; soybean, Glycine max L. Merr.

Dicamba residues in sprayers are difficult to remove and may interact with subsequent herbicides, including contact herbicides labeled for use in soybean. Without proper tank cleanout, applicators treating dicamba-resistant and non–dicamba resistant crops are at risk of contaminating the spray solution with dicamba residue from previous applications. Experiments were conducted in Fayetteville, AR, in 2018 and 2019, with the first experiment evaluating consequences of dicamba tank contamination with contact herbicides and the second experiment addressing the impact of dicamba exposure on a glufosinate-resistant soybean cultivar relative to a contact herbicide application. Experiments for tank contamination and timing of dicamba exposure were designed as a three-factor and a two-factor randomized complete block with four replications, respectively, considering site-year as a fixed effect in each experiment. Dicamba at 0, 0.056, 0.56, and 5.6 g ae ha^–1 was applied alone, with glufosinate, with acifluorfen, or with glufosinate plus acifluorfen to V3 soybean. Dicamba applied in combination with contact herbicides exacerbated visible auxin symptomology over dicamba alone at 21 and 28 d after treatment (DAT), while dicamba at 5.6 g ae ha^–1 reduced soybean height. Injury and height reductions caused by dicamba mixtures with contact herbicides did not reduce grain yield. In the second experiment, dicamba was applied at 2.8 g ae ha^–1 at VC, V1, V2, and V3 and at 3, 7, and 10 d after a glufosinate application to V3 soybean (DATV3). Greater soybean injury was observed when dicamba exposure followed a glufosinate application than when dicamba preceded glufosinate or was applied in a mixture with glufosinate, with yield reductions resulting from 7 and 10 DATV3 dicamba applications. Dicamba exposure in the presence of contact herbicides resulted in increased auxin symptomology and can be intensified if soybean are exposed to dicamba following a contact herbicide application.

Nomenclature: Acifluorfen; dicamba; glufosinate; soybean; Glycine max (L.) Merr.

Dicamba is a synthetic auxin herbicide that is prone to off-target movement, including drift and volatilization. Due to the increased acreage of dicamba-resistant soybean to control glyphosate-resistant weeds, dicamba drift injury to neighboring vegetable crops is of concern. A method to quantify leaf deformation (often referred to as leaf cupping) caused by dicamba injury was developed and compared to visual rating techniques to determine its accuracy and suitability. A second objective was to determine the relative dicamba sensitivity of several economically important vegetable crops. Soybean, snap bean, tomato, and cucumber were grown in a greenhouse and exposed to dicamba at 0, 56, 112, 280, 560, 1,120, and 2,240 mg ae ha^–1, which is, respectively, 0, 1/10,000, 1/5,000, 1/2,000, 1/1,000, 1/500, and 1/250 of the maximum recommended label rate for soybean application (560 g ae ha^–1). Plants were evaluated visually and using an imaging analysis technique that measures the leaf deformation index (LDI) with a leaf area scanner. LDI is calculated by dividing the two-dimensional projection of the area of the leaf in its natural configuration by the area of the flattened leaf. Across all four crops, log-logistic regression analysis indicated the LDI method had lower I₅₀ values with lower standard error, demonstrating that the LDI method gives more precise estimates of sensitivity. This novel method provides an objective, quantitative method for measuring dicamba drift injury and determining relative sensitivities of valuable specialty crops.

Nomenclature: dicamba; cucumber; Cucumis sativus L.; snap bean; Phaseolus vulgaris L.; soybean; Glycine max L. Merr.; tomato; Solanum lycopersicum L.

XtendFlex^® technology from Bayer allows growers to apply glyphosate, glufosinate, and dicamba POST to cotton. Since the evolution and spread of glyphosate-resistant weed species, early POST applications with several modes of action have become common. However, crop injury potential from these applications warrants further examination. Field studies were conducted from 2015 to 2017 at two locations in Mississippi to evaluate XtendFlex^® cotton injury from herbicide application. Herbicide applications were made to XtendFlex^® cotton at the three- to six-leaf stage with herbicide combinations composed of two-, three-, and four-way combinations of glyphosate, glufosinate, S-metolachlor, and three formulations of dicamba. Data collection included visual estimations of injury, stand counts, cotton height, total mainstem nodes, and nodes above whiteflower at first bloom. Data collection at the end of the season included cotton height, total mainstem nodes, and nodes above cracked boll. Visual estimations of injury from herbicide applications were highest at 3 d following applications containing glufosinate + S-metolachlor (36% to 41% injury) and glufosinate + S-metolachlor in combination with dicamba + glyphosate (39% to 41% injury), regardless of the dicamba formulation. Crop injury decreased at each rating interval and dissipated by 28 d following applications (P = 0.3748). Height reductions were present at first bloom and at the end of the season (P < 0.0001), although cotton yield was unaffected (P = 0.2089), even when injury at 3 d after application was greater than 30%. Results indicate that growers may apply a variety of herbicide tank mixtures to XtendFlex^® cotton and expect no yield penalty. Furthermore, if growers are concerned with cotton injury after herbicide applications, the use of glufosinate in combination with S-metolachlor should be approached with caution in XtendFlex^® cotton.

Nomenclature: Dicamba; glufosinate; glyphosate; S-metolachlor; cotton; Gossypium hirsutum L.

A field study was conducted in Mississippi to determine the effect of reduced dicamba rates on sweetpotato crop tolerance and storage root yield, simulating off-target movement or sprayer tank contamination. Treatments included a nontreated control and four rates of dicamba [70 g ae ha^–1 (1/8×), 35 g ae ha^–1 (1/16×), 8.65 g ae ha^–1 (1/64×), and 1.09 g ae ha^–1 (1/512×)] applied either 3 d before transplanting (DBP) or 1, 3, 5, or 7 wk after transplanting (WAP). An additional treatment consisted of 560 g ae ha^–1 (1×) dicamba applied 3 DBP. Crop injury ratings were taken 1, 2, 3, and 4 wk after treatment (WAT). Across application timings, predicted sweetpotato plant injury 1, 2, 3, and 4 WAT increased from 3T to 22%, 3% to 32%, 2% to 58%, and 1% to 64% as dicamba rate increased from 0 to 70 g ha^–1 (1/8×), respectively. As dicamba rate increased from 1/512× to 1/8×, predicted No. 1 yield decreased from 127% to 55%, 103% to 69%, 124% to 31%, and 124% to 41% of the nontreated control for applications made 1, 3, 5, and 7 WAP, respectively. Similarly, as dicamba rate increased from 1/512× to 1/8×, predicted marketable yield decreased from 123% to 57%, 107% to 77%, 121% to 44%, and 110% to 53% of the nontreated control for applications made 1, 3, 5, and 7 WAP, respectively. Dicamba residue (5.3 to 14.3 parts per billion) was detected in roots treated with 1/16× or 1/8× dicamba applied 5 or 7 WAP and 1/64× dicamba applied 7 WAP with the highest residue detected in roots harvested from sweetpotato plants treated at 7 WAP. Collectively, care should be taken to avoid sweetpotato exposure to dicamba especially at 1/8× and 1/16× rates during the growing season.

Nomenclature: Dicamba; sweetpotato; Ipomoea batatas (L.) Lam. ‘Beauregard’

Control of waterhemp is becoming more difficult in Ontario because biotypes have evolved resistance to four herbicide sites of action (SOA), including groups 2, 5, 9, and 14. The objective of this study was to compare PRE, POST, and PRE followed by (fb) POST herbicide programs for their effect on control, density, and biomass of multiple-herbicide–resistant (MHR) waterhemp as well as corn injury and grain yield. Two separate field studies, each consisting of five field trials, were conducted over a 2-yr period (2018 and 2019) in fields where corn was grown in Ontario, Canada. The first experiment evaluated MHR waterhemp control with an inhibitor of 4-hydroxyphenyl-pyruvate dioxygenase (HPPD) applied PRE, PRE fb glufosinate applied POST, and glufosinate applied POST. The second experiment evaluated MHR waterhemp control with a non-HPPD inhibitor applied PRE, then PRE fb a POST application of atrazine + mesotrione, and then atrazine + mesotrione applied POST. Atrazine + isoxaflutole caused 3% to 5% corn injury at environment 1 (E1); no corn injury was observed with PRE and POST herbicide programs at environments E2, E3, E4, and E5. In general, atrazine/bicyclopyrone/mesotrione/S-metolachlor and dimethenamid-P/saflufenacil applied PRE controlled MHR waterhemp ≥95% 12 wk after POST application (WAA). A POST application of glufosinate following atrazine + tolpyralate PRE, and a POST application of atrazine + mesotrione following atrazine/dicamba or atrazine/Smetolachlor PRE, improved control at 4, 8, and 12 WAA in most environments. In general, PRE fb POST applications resulted in better control of MHR waterhemp throughout the growing season than single PRE and POST applications (P < 0.05). We conclude that herbicide programs based on multiple effective SOAs may offer effective control of MHR waterhemp where field corn is grown. It is advisable that when choosing an herbicide application program that excellent control of MHR waterhemp should be the goal given its high fecundity and competitive ability.

Nomenclature: Atrazine; bicyclopyrone; dimethenamid-P; glufosinate; isoxaflutole; mesotrione; saflufenacil; S-metolachlor; tolpyralate; corn; Zea mays L.; waterhemp; Amaranthus tuberculatus (Moq.) J. D. Sauer

Velvetleaf is an economically important weed in agronomic crops in Nebraska and the United States. Dicamba applied alone usually does not provide complete velvetleaf control, particularly when velvetleaf is taller than 15 cm. The objectives of this experiment were to evaluate the interaction of dicamba, fluthiacet-methyl, and glyphosate applied alone or in a mixture in two- or three-way combinations for velvetleaf control in dicamba/glyphosate–resistant (DGR) soybean and to evaluate whether velvetleaf height (≤12 cm or ≤20 cm) at the time of herbicide application influences herbicide efficacy, velvetleaf density, biomass, and soybean yield. Field experiments were conducted near Clay Center, NE in 2019 and 2020. The experiment was arranged in a split-plot with velvetleaf height (≤12 cm or ≤20 cm) as the main plot treatment and herbicides as subplot treatment. Fluthiacet provided ≥94% velvetleaf control 28 d after treatment (DAT) and ≥96% biomass reduction regardless of application rate or velvetleaf height. Velvetleaf control was 31% to 74% at 28 DAT when dicamba or glyphosate was applied alone to velvetleaf ≤20 cm tall compared with 47% to 100% control applied to ≤12-cm-tall plants. Dicamba applied alone to ≤20-cm-tall velvetleaf provided <75% control and <87% biomass reduction 28 DAT compared with ≥90% control with dicamba at 560 g ae ha^–1 + fluthiacet at 7.2 g ai ha^–1 or glyphosate at 1,260 g ae ha^–1. Dicamba at 280 g ae ha^–1 + glyphosate at 630 g ae ha^–1 applied to ≤20-cm-tall velvetleaf resulted in 86% control 28 DAT compared with the expected 99% control. The interaction of dicamba + fluthiacet + glyphosate was additive for velvetleaf control and biomass reduction regardless of application rate and velvetleaf height.

Nomenclature: Dicamba; fluthiacet-methyl; glyphosate; velvetleaf; Abutilon theophrasti Medik.; soybean; Glycine max (L.) Merr.

In current and next-generation weed control technologies, sequential applications of contact and systemic herbicides for postemergence control of troublesome weeds are needed to mitigate the evolution of herbicide resistance. A clear understanding of the impact auxin herbicide symptomology has on Palmer amaranth groundcover will aid optimization of sequential herbicide applications. Field and greenhouse experiments were conducted in Fayetteville, AR, and a laboratory experiment was conducted in Lonoke, AR, in 2020 to evaluate changes in Palmer amaranth groundcover following an application of 2,4-D and dicamba with various nozzles, droplet sizes, and velocities. Field experiments utilized three nozzles: Extended Range (XR), Air Induction Extended Range (AIXR), and Turbo TeeJet^® Induction (TTI), to assess the effect of spray droplet size on changes in Palmer amaranth groundcover. Nozzle did not affect Palmer amaranth groundcover when dicamba was applied. However, nozzle selection did impact groundcover when 2,4-D was applied; the following nozzle order XR > AIXR > TTI reduced Palmer amaranth groundcover the most in both site-years of the field experiment. This result (XR > AIXR > TTI) matches percent spray coverage data for 2,4-D and is inversely related to spray droplet size data. Rapid reductions of Palmer amaranth groundcover from 100% at time zero to 39.4% to 64.1% and 60.0% to 85.8% were observed 180 min after application in greenhouse and field experiments, respectively, regardless of herbicide or nozzle. In one site-year of the greenhouse and field experiments, regrowth of Palmer amaranth occurred 10,080 min (14 d) after an application of either 2,4-D or dicamba to larger than labeled weeds. In all experiments, complete reduction of live Palmer amaranth tissue was not observed 21 d after application with any herbicide or nozzle combination. Control of Palmer amaranth escapes with reduced groundcover may potentially lead to increased selection pressure on sequentially applied herbicides due to a reduction in spray solution contact with the targeted pest.

Nomenclature: 2; 4-D; dicamba; Palmer amaranth, Amaranthus palmeri (S.) Watson

Palmer amaranth is the most problematic and troublesome weed in agronomic cropping systems in the United States. Acetolactate synthase (ALS) inhibitor and glyphosate-resistant (GR) Palmer amaranth has been confirmed in Nebraska and it is widespread in several counties. Soybean resistant to isoxaflutole/glufosinate/glyphosate has been developed that provides additional herbicide site of action for control of herbicide-resistant weeds. The objectives of this study were to evaluate herbicide programs for control of ALS inhibitor/GR Palmer amaranth and their effect on Palmer amaranth density and biomass, as well as soybean injury and yield in isoxaflutole/glufosinate/glyphosate–resistant soybean. Field experiments were conducted in a grower's field infested with ALS inhibitor and GR Palmer amaranth near Carleton, Nebraska, in 2018 and 2019. Isoxaflutole applied alone or mixed with sulfentrazone/pyroxasulfone, flumioxazin/pyroxasulfone, or imazethapyr/saflufenacil/pyroxasulfone provided similar control (86%–99%) of Palmer amaranth 21 d after PRE (DAPRE). At 14 d after early-POST (DAEPOST), isoxaflutole applied PRE and PRE followed by (fb) POST controlled Palmer amaranth by 10% to 63% compared to 75% to 96% control with glufosinate applied EPOST in both years. A PRE herbicide fb glufosinate controlled Palmer amaranth 80% to 99% 21 d after late-POST (DALPOST) in 2018, and reduced density 89% to 100% in 2018 and 58% to 100% in 2019 at 14 DAEPOST. No soybean injury was observed from any of the herbicide programs tested in this study. Soybean yield in 2019 was relatively higher due to higher precipitation compared with 2018 with generally no differences between herbicide programs. This research indicates that herbicide programs are available for effective control of ALS inhibitor/GR Palmer amaranth in isoxaflutole/glufosinate/glyphosate-resistant soybean.

Nomenclature: Flumioxazin; glufosinate; glyphosate; imazethapyr; isoxaflutole; pyroxasulfone; saflufenacil; sulfentrazone; Palmer amaranth; Amaranthus palmeri S. Watson; soybean; Glycine max (L.) Merr.

Laboratory and greenhouse studies were conducted to evaluate the effects of chemical treatments applied to Palmer amaranth seeds or gynoecious plants that retain seeds to determine seed germination and quality. Treatments applied to physiologically mature Palmer amaranth seed included acifluorfen, dicamba, ethephon, flumioxazin, fomesafen, halosulfuron, linuron, metribuzin, oryzalin, pendimethalin, pyroxasulfone, S-metolachlor, saflufenacil, trifluralin, and 2,4-D plus crop oil concentrate applied at 1× and 2× the suggested use rates from the manufacturer. Germination was reduced by 20% when 2,4-D was used, 15% when dicamba was used, and 13% when halosulfuron and pyroxasulfone were used. Use of dicamba, ethephon, halosulfuron, oryzalin, trifluralin, and 2,4-D resulted in decreased seedling length by an average of at least 50%. Due to the observed effect of dicamba, ethephon, halosulfuron, oryzalin, trifluralin, and 2,4-D, these treatments were applied to gynoecious Palmer amaranth inflorescence at the 2× registered application rates to evaluate their effects on progeny seed. Dicamba use resulted in a 24% decrease in seed germination, whereas all other treatment results were similar to those of the control. Crush tests showed that seed viability was greater than 95%, thus dicamba did not have a strong effect on seed viability. No treatments applied to Palmer amaranth inflorescence affected average seedling length; therefore, chemical treatments did not affect the quality of seeds that germinated.

Nomenclature: acifluorfen; dicamba; ethephon; flumioxazin; fomesafen; halosulfuron; linuron; metribuzin; oryzalin; pendimethalin; pyroxasulfone; S-metolachlor; saflufenacil; trifluralin; 2,4-D; Palmer amaranth, Amaranthus palmeri S. Watson; AMAPA

Glyphosate-resistant (GR) Palmer amaranth is one of the most difficult to control weeds in soybean production fields in Nebraska and the United States. An integrated approach is required for effective management of GR Palmer amaranth. Cultural practices such as narrow row spacing might augment herbicide efficacy for management of GR Palmer amaranth. The objectives of this study were to evaluate the effect of row spacing and herbicide programs for management of GR Palmer amaranth in dicamba/glyphosate-resistant (DGR) soybean. Field experiments were conducted in a grower's field with a uniform population of GR Palmer amaranth near Carleton, Nebraska, in 2018 and 2019. Year-by-herbicide program-by-row spacing interactions were significant for all variables; therefore, data were analyzed by year. Herbicides applied PRE controlled GR Palmer amaranth ≥95% in both years 14 d after PRE (DAPRE). Across soybean row-spacing, most PRE followed by (fb) early-POST (EPOST) herbicide programs provided 84% to 97% control of Palmer amaranth compared with most EPOST fb late-post (LPOST) programs, excluding dicamba in single and sequential applications (82% to 95% control). Mixing microencapsulated acetochlor with a POST herbicide in PRE fb EPOST herbicide programs controlled Palmer amaranth ≥93% 14 d after EPOST and ≥96% 21 d after LPOST with no effect on Palmer amaranth density. Interaction of herbicide program-by-row spacing on Palmer amaranth control was not significant; however, biomass reduction was significant at soybean harvest in 2019. The herbicide programs evaluated in this study caused no soybean injury. Due to drought conditions during a majority of the 2018 growing season, soybean yield in 2018 was reduced compared with 2019.

Nomenclature: Acetochlor; chlorimuron; dicamba; glyphosate; imazethapyr; flumioxazin; fomesafen; metribuzin; pyroxasulfone; saflufenacil; Smetolachlor; Palmer amaranth; Amaranthus palmeri S. Watson; soybean; Glycine max (L.) Merr.

Palmer amaranth–a fast-growing, challenging-to-control noxious weed that significantly reduces crop yields—was first found in Minnesota in September 2016 in conservation plantings sown with Palmer amaranth contaminated seed mixes. Minnesota Department of Agriculture (MDA) designated Palmer amaranth as a Prohibited Noxious Weed in 2015 and listed it as a Noxious Weed Seed in 2016 by emergency order. A genetic test to identify Palmer amaranth was simultaneously developed by multiple laboratories, providing a tool to limit its spread as a contaminant in seed. Seed companies adopted genetic testing methods for labeling seed for sale, thus reducing introductions via the seed pathway. Additionally, MDA determined that manure spread on crop fields from contaminated screenings fed to livestock resulted in new infestations. Limiting spread via these and other potential pathways was critical to successfully reducing the impact of Palmer amaranth. MDA, University of Minnesota (UMN) Extension, Conservation Corps Minnesota and Iowa (CCMI), farmers, and other partners are working to eradicate these infestations before they can spread. In 2016, 35 sites were sown with Palmer amaranth–contaminated seed mixes. Palmer amaranth was found at eight (23%) of these sites. Management with intensive scouting, torching, prescribed burning, and herbicide application was implemented in 2016 and 2017. By 2018, no Palmer amaranth was found at any of these sites. Similar success to newer infestations in 2018, 2019, and 2020 was achieved using the same methods. MDA recorded management activities and documented a comprehensive timeline of Palmer amaranth in Minnesota. This timeline provides a story of success and challenges in combating and eradicating Palmer amaranth.

Nomenclature: Palmer amaranth; Amaranthus palmeri S. Watson

Glyphosate-resistant (GR) horseweed was first confirmed in Ontario in 2010. GR horseweed interference can reduce soybean yield by up to 97%. Bromoxynil is a photosystem II–inhibiting herbicide that is primarily used for annual broadleaf weed control in monocot crops. The objective of this study was to determine the biologically effective dose (BED) of bromoxynil applied alone and when mixed with metribuzin applied preplant for control of GR horseweed in soybean in Ontario. Five field experiments were conducted over a 2-yr period (2019–2020) to determine the predicted dose of bromoxynil with or without metribuzin that would control GR horseweed 50%, 80%, and 95%. No soybean injury was observed. The predicted doses of bromoxynil to achieve 50% and 80% control of GR horseweed were 98 and 277 g ai ha^–1, respectively, at 8 wk after application (WAA). When mixed with metribuzin (400 g ai ha^–1), the predicted doses of bromoxynil for 50%, 80%, and 95% control of GR horseweed were 10, 25, and 54 g ai ha^–1, respectively. Bromoxynil (280 g ai ha^–1) plus metribuzin (400 g ai ha^–1) controlled GR horseweed 97%, a finding that was similar to the industry standards of saflufenacil + metribuzin (99% control) and glyphosate/dicamba + saflufenacil (100% control) at 8 WAA. This study concludes that bromoxynil + metribuzin applied before planting provides excellent control of GR horseweed in soybean.

Nomenclature: Bromoxynil; metribuzin; horseweed, Conyza canadensis (L.) Cronq.; soybean; Glycine max (L.) Merr.

Tiafenacil is a recently developed protoporphyrinogen IX oxidase (PPO)-inhibiting herbicide from the pyrimidinedione chemical class that is proposed for use as a preplant (PP) burndown in soybean. Glyphosate-resistant (GR) horseweed is a troublesome weed often found in no-till systems that can dramatically reduce soybean yield; control in soybean has been variable. Five field experiments were conducted over 2019 and 2020 in commercial soybean fields with GR horseweed to determine the biologically effective dose (BED) of tiafenacil and tiafenacil + metribuzin and to compare their efficacy to currently accepted industry standard herbicide treatments in identity-preserved (IP, non-GMO), GR, and glyphosate/dicamba-resistant (GDR) soybean systems. There was no soybean injury with treatments evaluated. The calculated doses of tiafenacil for 50%, 80%, and 95% control of GR horseweed control were 21, 147, and >200 g ai ha^–1, respectively, at 8 wk after application (WAA). Lower doses were calculated with the addition of metribuzin (400 g ai ha^–1) to tiafenacil for 50% and 80% control, with no dose of tiafenacil + metribuzin providing 95% control. Tiafenacil + metribuzin at 25 + 400 and 50 + 400 g ai ha^–1 controlled GR horseweed 88% and 93%, respectively, which was similar to the industry standards of saflufenacil + metribuzin (25 + 400 g ai ha^–1) and glyphosate/dicamba + saflufenacil (1,200/600 + 25 g ai ha^–1) that provided 98% and 100% control, respectively, at 8 WAA. This study presents the potential utility of tiafenacil + metribuzin as a GR horseweed management strategy in soybean.

Nomenclature: Dicamba; glyphosate; metribuzin; saflufenacil; tiafenacil; horseweed; Conyza canadensis (L.) Cronquist; soybean; Glycine max (L.) Merr.

Glyphosate resistance in weed species has presented immense challenges for farmers in Ontario. The co-application of burndown plus residual herbicides provides control of glyphosate-resistant (GR) horseweed in soybean. Pyraflufen-ethyl/2,4-D is a premixed herbicide formulation sold under the tradename Blackhawk^®. Five field experiments were conducted over a 2-yr period (2019, 2020) in fields in southwestern Ontario to ascertain the biologically effective dose of pyraflufen-ethyl/2,4-D, applied alone, or mixed with metribuzin, for GR horseweed control when applied preplant to soybean. Soybean visible injury for all treatments was <15%. At 8 wk after application (WAA), the calculated doses of pyraflufen-ethyl/2,4-D for 50%, 80%, and 95% control of GR horseweed were 390, 1,148, and >2,108 g ha^–1, respectively. The addition of metribuzin to pyraflufen-ethyl/2,4-D reduced the doses of pyraflufen-ethyl/2,4-D for 50%, 80%, and 95% control of GR horseweed to 19, 46, and 201 g ha^–1, respectively. Pyraflufen-ethyl/2,4-D + metribuzin controlled GR horseweed by 97%, which is comparable to the current industry standards. Based on these results, pyraflufen-ethyl/2,4-D + metribuzin (527 + 400 g ha^–1) applied preplant can be used for GR horseweed control in soybean.

Nomenclature: 2,4-D; metribuzin; pyraflufen-ethyl; horseweed; Conyza canadensis (L.) Cronq.; soybean; Glycine max (L.) Merr

Amid widespread occurrence of herbicide-resistant weeds in the United States, the use of PRE herbicides and cover crops have resurged once again as important strategies for weed management in cropping systems. The objective of this experiment was to evaluate the length of soil residual weed control from PRE soybean herbicides and the detrimental impact of these herbicides on cover crop species using field treated soil in greenhouse bioassays. Greenhouse bioassays were conducted using soil from field experiments conducted in 2018 and 2019 in Arlington and Lancaster, WI. PRE herbicides consisted of imazethapyr, chlorimuron-ethyl, and cloransulam-methyl (acetolactate synthase [ALS] inhibitors); metribuzin (photosystem II [PS II] inhibitor); sulfentrazone, flumioxazin, and saflufenacil (protoporphyrinogen oxidase [PPO] inhibitors); acetochlor, S-metolachlor, dimethenamid-P, and pyroxasulfone (very long-chain fatty acid [VLCFA] inhibitors); and a nontreated control. Greenhouse bioassays were conducted using soil (depth, 0 to 10 cm) sampled at 0, 10, 20, 30, 40, and 50 d after treatment (DAT). Palmer amaranth and giant foxtail (weeds), and radish and cereal rye (cover crops) were used as bioindicators of herbicide levels in the soil. Bioassay results showed extended soil residual control of Palmer amaranth with sulfentrazone and pyroxasulfone; extended residual control of giant foxtail was observed with pyroxasulfone and S-metolachlor. Chlorimuron-ethyl and metribuzin were the most injurious herbicides to radish and cereal rye shortly after application, respectively, but minimal injury was observed from soil samples collected 50 DAT, indicating the use of PRE and fall-seeded cover crops in southern Wisconsin can be compatible. These results can support growers and practitioners with selection of effective PRE herbicides for Palmer amaranth and giant foxtail control and reduced impact on fall-seeded radish and cereal rye cover crops, altogether leading to more effective, diverse, and sustainable weed management programs.

Nomenclature: Acetochlor; chlorimuron-ethyl; cloransulam-methyl; dimethenamid-P; flumioxazin; imazethapyr; metribuzin; pyroxasulfone; saflufenacil; S-metolachlor; sulfentrazone; giant foxtail; Setaria faberi Herrm.; Palmer amaranth; Amaranthus palmeri S. Watson; cereal rye; Secale cereale L.; radish; Raphanus sativus L.; soybean; Glycine max (L.) Merrill

The evolution of herbicide-resistant weeds has resulted in the necessity to integrate nonchemical control methods with chemicals for effective management in crop production systems. In soybean, control of the pigweed species, particularly herbicide-resistant waterhemp and Palmer amaranth, have become predominant concerns. Cereal rye planted as a winter cover crop can effectively suppress early-season weed emergence in soybean, including waterhemp, when planted at a rate of 123 kg ha^–1. The objectives of this study were to determine the effects of different cereal rye seeding rates (0, 34, 56, 79, 110, and 123 kg ha^–1) on early-season waterhemp suppression and soybean growth and yield. Soybean was planted into fall-seeded cereal rye, which was terminated within 4 d of soybean planting. The experiment was conducted over the 2018, 2019, and 2020 growing seasons in Columbia, Missouri. Effects of cereal rye on early-season waterhemp suppression varied by year and were most consistent at 56 kg ha^–1 or higher seeding rates. Linear regression analysis of cereal rye biomass, height, or stand at soybean planting showed inverse relationships with waterhemp emergence. No adverse effects on soybean growth or yield were observed at any of the cereal rye seeding rates relative to plots that lacked cereal rye cover. Result differences among the years suggest that the successfulness of cereal rye on suppression of early-season waterhemp emergence is likely influenced by the amount of waterhemp seed present in the soil seed bank.

Nomenclature: Palmer amaranth; Amaranthus palmeri S. Wats.; waterhemp; Amaranthus tuberculatus (Moq) Sauer; cereal rye; Secale cereale L.; soybean; Glycine max (L.) Merr.

With the increasing focus on herbicide-resistant weeds and the lack of introduction of new modes of action, many producers have turned to planting annual cover crops as a method for reducing weed populations. Recent studies have suggested that perennial cover crops such as white clover could be used as living mulch. However, white clover is slow to establish and is susceptible to competition from winter weeds. Therefore, the objective of this study was to determine clover tolerance and weed control in established stands of white clover to several herbicides. Studies were conducted in the fall and winter of 2018 to 2019, and 2019 to 2020, at the J. Phil Campbell Research and Education Center in Watkinsville, GA, and the Southeast Georgia Research and Education Center in Midville, GA. POST applications of imazethapyr, bentazon, or flumetsulam at low and high rates, or in combination with 2,4-D and 2,4-DB, were applied when clover reached the 2- to 3-trifoliate stage. Six weeks after the initial POST application, a sequential application of bentazon and flumetsulam individually, and combinations of 2,4-D, 2,4-DB, and flumetsulam, were applied over designated plots. Clover biomass was similar across all treatments except where it was reduced by sequential applications of 2,4-D + 2,4-DB + flumetsulam in the 2019 to 2020 season, indicating that most treatments were safe for use on establishing living mulch clover. A single application of flumetsulam at the low rate or a single application of 2,4-D + 2,4-DB provided the greatest control of all weed species while minimizing clover injury compared with the nontreated check. These herbicide options allow for control of problematic winter weeds during clover establishment, thereby maximizing clover biomass and limiting canopy gaps that would allow summer weed emergence.

Nomenclature: 2,4-D; 2,4-DB; bentazon; flumetsulam; imazethapyr; common vetch; Vicia sativa L.; VISA; cutleaf evening primrose; Oenothera laciniata Hill; OELA; white clover; Trifolium repens L.; TRFRE; wild radish; Raphanus raphanistrum; RAPRA

Living mulches are cover crops grown simultaneously with and in close proximity to cash crops. Advantages of living mulches over dead cover crops may include increased weed suppression, reduced erosion and leaching, better soil health, and greater resource-use efficiency. Advantages of living mulches over synthetic mulches may include enhanced agroecosystem biodiversity and suitability for a wider range of cropping systems. A major disadvantage of this practice is the potential for competition between living mulches and cash crops. The intensity and outcome of mulch-crop competition depend on agroecosystem management as well as climate and other factors. In this review, we consider the management of living mulches for weed control in field and vegetable cropping systems of temperate environments. More than 50 yr of research have demonstrated that mechanical or chemical suppression of a living mulch can limit mulch-crop competition without killing the mulch and thereby losing its benefits. Such tactics can also contribute to weed suppression. Mechanical and chemical regulation should be combined with cultural practices that give the main crop a competitive advantage over the living mulch, which, in turn, outcompetes the weeds. Promising approaches include crop and mulch cultivar selection; changes to planting time, density, and planting pattern; and changes to fertilization or irrigation regimes. A systems approach to living mulch management, including an increased emphasis on the interactions between management methods, may increase the benefits and lower the risks associated with this practice.