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1 April 2009 Assessing Cheatgrass (Bromus tectorum) Genetic Diversity and Population Structure Using Rapd and Microsatellite Molecular Markers
Michael C. Ashley, William S. Longland
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Two molecular marker systems, random amplified polymorphic DNA (RAPD) and microsatellites, were used to evaluate population diversity and differentiation in 4 northern Nevada Bromus tectorum populations. From 80 RAPD primers, we found 16 (20%) that yielded 165 strong repeatable bands. Of those bands, 60 (35.8%) were polymorphic. Of those, 21 met data-pruning guidelines for final analysis. RAPD variation was moderate (fi01_63.gif = 0.363, Sfi02_63.gif = 0.022), ranging from 0.312 to 0.404. Microsatellite variation was similar (fi01_63.gif = 0.234, Sfi02_63.gif = 0.051) but varied more widely, ranging from 0.009 to 0.551. All populations were out of Hardy-Weinberg equilibrium, as expected in a predominantly selfing species. RAPDs revealed significant differentiation (P < 0.0001) across populations, whereas microsatellites only resolved 2 of the 4 populations. RAPDs revealed a considerable amount of variation in the 2 populations, Hot Springs and Truckee, which had nearly identical microsatellite profiles. Of 184 individuals, we found 182 unique RAPD phenotypes. We found 51 microsatellite genotypes across individuals, one of which was present in 95.3% of the individuals in the Hot Springs-Truckee group and 29% of the Stillwater individuals. The UPGMA phenograms were similar in grouping Hot Springs with Truckee, and Stillwater with Peavine, and were highly correlated but not significantly. Both marker systems proved useful in assessing population genetic variation. One population-specific RAPD marker and 6 new microsatellite-length polymorphisms were identified. Both marker systems may also prove useful in studying other closely related species such as red brome (Bromus nibens).

© 2009
Michael C. Ashley and William S. Longland "Assessing Cheatgrass (Bromus tectorum) Genetic Diversity and Population Structure Using Rapd and Microsatellite Molecular Markers," Western North American Naturalist 69(1), 63-74, (1 April 2009).
Received: 8 August 2007; Accepted: 1 September 2008; Published: 1 April 2009

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