Five millennia ago, the North American beaver (Castor canadensis) was extirpated from the Southern High Plains by droughts. Beaver were found to be largely absent from the Llano Estacado, despite exhaustive search efforts. Here we report the first definitive evidence of an extant C. canadensis population recolonizing the Llano Estacado. We further provide some spatiotemporal background information about beaver colonization along the North Fork of the Double Mountain Fork of the Brazos River.