Thyroid hormone receptors (TRs) are ligand-dependent transcription factors that regulate the transcription of multiple thyroid hormone (TH)-responsive genes. Our study aimed to identify TH-responsive genes in an estrogen-responsive chicken hepatoma cell line, LMH. RNA was prepared from cells treated with or without 10⁻⁸ M 3,3′,5-triiodothyronine (T₃) for 24 h and was analyzed by differential display. At least six cDNAs were detected whose transcript increased in the presence of T₃, and four of them were cloned. The four candidate TH-responsive genes that were identified had high similarity (>83%) to known chicken or mammalian genes, which included the ribosomal protein L7 gene; the cytoplasmic dynein heavy chain gene; the scaffold attachment factor A (SAF-A) gene, also known as heterogenous nuclear ribonucleoprotein U (hnRNP U); and a gene for an unknown protein. Real-time PCR confirmed that the transcription of the four genes was responsive to T₃; their transcript levels increased from four to eleven times with the administration of T₃. The amount of TRβ transcript did not change with the administration of T₃. The physiological reasons for the activation of these genes and the utility of this cell line are discussed.