Translator Disclaimer
1 December 2006 Perforin-Dependent Cell Death in Skin Allograft Rejection of the Terrestrial Slug Incilaria fruhstorferi
Emiko Furuta, Naomi Seo, Keiichiro Yamaguchi
Author Affiliations +
Abstract

The rejection of allografts in mammals is mainly mediated by cytotoxic T-lymphocytes, whereas no comparable immunoreactive cells have been described in invertebrates. The present study was undertaken to determine whether similar cytotoxic effector cells are present when allograft rejection occurs in the terrestrial slug Incilaria fruhstorferi. A piece of dorsal skin from a donor animal was orthotopically transplanted to a recipient. Immunohistochemistry for perforin, detection of apoptosis by the TUNEL (TdT-mediated dUTP-biotin nick-end labeling) method, and electron microscopy were performed using both donor and recipient tissues. Cellular changes in the rejection process continued over for 40 days. Two functional types of “effector” cells were recognized at the rejection site, but they were observed to be macrophages possessing perforin granules and phagocytosing damaged cells of the allograft. Three days after transplantation, the perforin-positive cells were recognized only in the recipient tissue surrounding the allograft. Five days after transplantation, these cells started to appear in the graft, while they disappeared from the host tissue. However, TUNEL-positive cells were not observed throughout the graft-rejection process. Electron microscopic examination of the graft tissue revealed autophagic degeneration of epithelial cells, mucous cells, pigment cells, fibroblasts, and muscle cells. These observations suggest that the molluscan slug has the capability to recognize differences in cell-surface molecules between the allogeneic and recipient tissues, and that an allograft is chronically rejected due to a type of immunocyte that can induce perforin-dependent cell death.

Emiko Furuta, Naomi Seo, and Keiichiro Yamaguchi "Perforin-Dependent Cell Death in Skin Allograft Rejection of the Terrestrial Slug Incilaria fruhstorferi," Zoological Science 23(12), 1093-1100, (1 December 2006). https://doi.org/10.2108/zsj.23.1093
Received: 6 September 2005; Accepted: 1 August 2006; Published: 1 December 2006
JOURNAL ARTICLE
8 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top