The aim of the present work is to conduct a comparative study of oxidative states in the nymphs of four species of Plecoptera belonging to the superfamily Perloidea: Perla marginata (Panzer, 1799) (family Perlidae), Guadalgenus franzi (Aubert, 1963), Isoperla curtate Navás, 1924, and lsoperla grammatica (Poda, 1761) (family Perlodidae) in relation to their ecological and biological characteristics. For this, the activity of the following antioxidant enzymes was determined: superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione transferase (GST), and DT-diaphorase (DTD), together with lipid peroxidation. Glucose 6-phosphate dehydrogenase (G6PDH) was also determined. The four species studied were selected based on significant ecological and biological differences. The results obtained when studying different indicative parameters of the oxidative state of the nymph of different species showed that each has an important enzymatic antioxidant potential, and that differences among species are conditioned by the duration of the nymphal development period more than by whether they come from permanent or temporary habitats. Thus, Plecoptera, although traditionally considered as typical inhabitants of permanent waters, seem to have sufficient variability in physiological mechanisms, together with behavioral and ecological adaptations, to cope with potentially unfavorable conditions that may occur in temporary waters.