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1 February 2011 Detection of Elevated Antibody Against Calreticulin by ELISA in Aged Cynomolgus Monkey Plasma
Atsunori Higashino, Takashi Kageyama, Sachi Sri Kantha, Keiji Terao
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Abstract

Calreticulin (Crt) is a molecular chaperone ubiquitously present in the endoplasmic reticulum. In non-human primates, age-related occurrence of anti-Crt antibody has not been reported. We developed an ELISA assay for an anti-Crt antibody and determined the age-related increase in the levels of anti-Crt antibody in three groups of cynomolgus monkeys: juvenile (1.5 yr), young adults (5–10 yr) and aged adults (20–34 yr). Mean ± SD auto-antibody levels at 450 nm in juvenile, young adults and aged groups were 0.23 ± 0.18, 0.30 ± 0.28, and 0.55 ± 0.33, respectively. Statistically significant differences were noted in the autoantibody levels to Crt among the aged group and juvenile or young adults. This is the first report to demonstrate the expression of anti-Crt autoantibody in aged monkeys and indicates that cynomologous monkeys may serve as an appropriate nonhuman primate model for studies of age-related alteration of immune function in elderly humans. Though preliminary, this finding merits further investigation to determine the relationship between immunosenescence and expression of antibodies to Crt.

© 2011 Zoological Society of Japan
Atsunori Higashino, Takashi Kageyama, Sachi Sri Kantha, and Keiji Terao "Detection of Elevated Antibody Against Calreticulin by ELISA in Aged Cynomolgus Monkey Plasma," Zoological Science 28(2), 85-89, (1 February 2011). https://doi.org/10.2108/zsj.28.85
Received: 1 April 2010; Accepted: 1 August 2010; Published: 1 February 2011
JOURNAL ARTICLE
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KEYWORDS
aging
autoantibody
calreticulin
Macaca fascicularis
suppressive/regulatory T cells
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