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1 September 2012 Molecular Cloning and Functional Characterization of Novel Antimicrobial Peptides from the Skin of Brown Frog, Rana zhenhaiensis
Baohua Xu, Helong Che, Lumei Kang, Shuangyan Zheng, Songniu Mu, Fusheng Wan
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Abstract

Rana zhenhaiensis, a species of brown frog, is widely distributed in central and south China. In the present study, a total of 14 cDNA sequences encoding eight novel antimicrobial peptides (AMPs) were cloned from the synthesized cDNAs of R. zhenhaiensis skin. The eight novel AMPs belong to four families: brevinin-1 (four peptides), brevinin-2 (one peptide), ranatuerin-2 (one peptide) and chensinin-1 (two peptides), five AMPs from the four families (brevinin-1ZHa, brevinin-1ZHb, brevinin-2ZHa, ranatuerin-2ZHa and chensinin-1ZHa) were chemically synthesized, their antimicrobial and hemolytic activities were examined. The results indicated that the five AMPs possess different antimicrobial and hemolytic activities. Of these, brevinin-2ZHa exhibited the strongest and most broad-spectrum antimicrobial activity. Furthermore, scanning electron microscopy (SEM) experiment was carried out to investigate the potential antimicrobial mechanism of chensinin-1ZHa. The result indicated that chensinin-1ZHa may exert its function through disruption of the bacterial membrane.

© 2012 Zoological Society of Japan
Baohua Xu, Helong Che, Lumei Kang, Shuangyan Zheng, Songniu Mu, and Fusheng Wan "Molecular Cloning and Functional Characterization of Novel Antimicrobial Peptides from the Skin of Brown Frog, Rana zhenhaiensis," Zoological Science 29(9), 553-558, (1 September 2012). https://doi.org/10.2108/zsj.29.553
Received: 21 March 2012; Accepted: 6 May 2012; Published: 1 September 2012