Twenty-two novel cDNAs encoding 22 peptide precursors for 19 mature peptides including antimicrobial peptides (AMPs) were identified from East Asian frog species Babina daunchina, Babina adenopleura, and Rana omeimontis skin-derived cDNA libraries. Two atypical members of the brevinin-1 family AMPs, named brevinin-1AN1 (FLTGVLKLASKIPSVLCAVLKTC) and brevinin-1DN1(FLKGVINLASKIPSMLCAVLKTC), were purified from the skin secretions of B. adenopleura and B. daunchina, respectively. A member of the ranatuerin-2 family AMP named ranatuerin-2DN1 (GLFDSITQGLKDTAVKLLDKIKCKLSACPPA) was also purified from the skin secretion of B. daunchina. One AMP named japonicin-2OM1 (FIVPSIFLLKKAFCIALKKNC) was purified from the skin secretion of R. omeimontis. The antimicrobial tests showed that brevinin-1DN1, brevinin-1DN2, brevinin-1AN1, and japonicin-2OM1 possess higher antimicrobial activity against Gram-positive bacteria than Gram-negative bacteria.
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1 July 2014
Diverse Families of Antimicrobial Peptides Isolated from Skin Secretions of Three Species of East Asian Frogs, Babina daunchina, Babina adenopleura, and Rana omeimontis (Ranidae)
Yuhong Hu,
Shiqi Xu,
Yonghong Hu,
Chao Guo,
Hao Meng,
Jing Li,
Jingze Liu,
Hui Wang
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Zoological Science
Vol. 31 • No. 7
July 2014
Vol. 31 • No. 7
July 2014
Amphibia
brown frog
gene
peptide
Ranidae