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1 December 2018 Molecular Characterization and Tissue Distribution of Mitochondrial Ca2 -Dependent Solute Carrier Protein during Prevention of Diapause by HCl Treatment in the Silkworm, Bombyx mori
Ryo Kitta, Yumi Yamahama, Takayuki Yamamoto, Keisuke Mase, Hiroshi Sawada
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Abstract

To clarify the molecular mechanism of prevention of entry into diapause in Bombyx mori by HCl treatment, we biochemically analyzed mitochondrial Ca2 -dependent solute carrier protein (MCSC) in diapause eggs treated with HCl solution. Our previous studies revealed that HCl treatment causes Ca2 to efflux from diapause eggs. Therefore, we attempted to analyze MCSC, which is known to associate with Ca2 . The isolated cDNA of B. mori MCSC (BmMCSC) had an open reading flame (ORF) of 667 amino acid residues, and the ORF contained two EF-hand calcium-binding domains and three characteristic features of the mitochondrial solute carrier superfamily. The gene expression level of BmMCSC increased by HCl treatment. A Ca2 binding assay indicated that recombinant BmMCSC (rBmMCSC) shows an affinity with Ca2 . The distribution of BmMCSC was investigated with an immunohistochemical technique using antisera against BmMCSC in diapause eggs and HCl-treated diapause eggs. BmMCSC was localized in serosa cells in both eggs. These data may suggest that BmMCSC is activated by intracellular Ca2 or efflux Ca2 by HCl treatment, and that it plays a role in the molecular mechanisms of artificial diapause prevention or the breaking of diapause in the silkworm.

© 2018 Zoological Society of Japan
Ryo Kitta, Yumi Yamahama, Takayuki Yamamoto, Keisuke Mase, and Hiroshi Sawada "Molecular Characterization and Tissue Distribution of Mitochondrial Ca2 -Dependent Solute Carrier Protein during Prevention of Diapause by HCl Treatment in the Silkworm, Bombyx mori," Zoological Science 35(6), 487-493, (1 December 2018). https://doi.org/10.2108/zs180041
Received: 6 March 2018; Accepted: 26 June 2018; Published: 1 December 2018
KEYWORDS
Bombyx mori
Ca2
diapause
HCl treatment
MCSC
serosa cell
silkworm
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