Avian polyomavirus infection in psittacines was diagnosed in tissues by the use of polymerase chain reaction (PCR) test. The tissues used in the procedure were either formalin-fixed tissues embedded in paraffin blocks or fresh tissues (heart, liver, and spleen) collected from the psittacines during necropsy. DNA was extracted from these tissues and was tested with the published primers for avian polyomavirus VP1 gene in the PCR that yielded an amplicon of 550 base pair size, which was then visualized by electrophoresis. The amplicon size was consistent with avian polyomavirus. The PCR test was found to be an effective method for identifying avian polyomavirus infection in both formalin-fixed, paraffin-embedded and fresh tissues from psittacine birds of different age groups.
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1 April 2004
Detection of Avian Polyomavirus Infection by Polymerase Chain Reaction Using Formalin-Fixed, Paraffin-Embedded Tissues
Parimal Roy,
A. S. Dhillon,
Lloyd Lauerman,
H. L. Shivaprasad
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Avian Diseases
Vol. 48 • No. 2
April 2004
Vol. 48 • No. 2
April 2004
avian polyomavirus
formalin-fixed paraffin-embedded tissues
pathology
polymerase chain reaction