The endangered Indiana bat (Myotis sodalis) congregates in large hibernation groups in winter and travels after spring emergence to form summer maternity colonies, but information on migration behavior in this species remains limited to mostly band recovery observations. We tracked female Indiana bats in spring migration toward summer grounds using aerial radiotelemetry. Adult female Indiana bats were radiotagged in spring from 2009 through 2017, with 15 individuals successfully tracked to summer grounds and an additional 11 bats located in summer grounds via aerial telemetry after migration was complete. This resulted in the location of 17 previously unknown summer grounds for female Indiana bats, including adding Georgia, Alabama, and Mississippi to the summer maternity range. Two of the colonies identified in this study were south of the previously known southernmost colony in Tennessee, expanding the summer maternity range for the species by 178 km. Time-stamped location fixes along the migration path provided information about nightly and overall distances traveled, duration of travel, migration speed, and weather-related influences on bat behavior. Bats traveled 164.6 ± 26.2 km (± SE) on average from hibernacula to summer grounds and were migrating for an average of 7.3 ± 1.4 calendar nights. Bats alternated between foraging and traveling throughout each night of their migration route. Nightly migration rate was 9.9 ± 0.8 km/h and bats were active on the landscape for an average of 6.1 ± 0.4 h/night. Lower nighttime temperatures and lower barometric pressure correlated with use of layover areas during a migration night. Understanding bat behavior during migration can provide pertinent information for land managers to consider in efforts to conserve potential migration corridors, foraging areas, and roosting habitats of species in decline.