A survey of Coccinella septumpunctata on alfalfa Medicago sativa L. was carried out during spring in 2013 at Farafra and Bahariya Oases in Egypt. Insects were collected from six different regions located in the two oases. Genetic diversity within the species was accessed by using five RAPD markers. A total of 55 fragments were amplified, of which 48 fragments were polymorphic showing 87.3 % polymorphism. The number of amplification products ranged from nine to 15 with an average of 12 per primer. The maximum of polymorphic bands (15 bands) belonged to K15 primer, whereas the minimum of polymorphic bands (7 bands) belonged to C15 primer. Genetic characterization was done with the help of similarity matrix. The similarity of DNA bands ranged from 0.578 to 0.774.