The encroachment of agricultural landscapes on natural ecosystems poses a significant threat to native wildlife persistence. In the Boland Mountain Complex (BMC), the presence of mammals was well documented in the 20th century, but an updated account is lacking. This study relied on Local Ecological Knowledge (LEK) to elucidate perceived medium- to large-sized (>300 g) mammal occurrences, distribution ranges, and relative abundance on private agricultural properties adjacent to protected areas in the BMC. In total, 30 mammal species were reported to be present, and inverse distance weighted (IDW) interpolations were created for species that had more than five independent citations (n = 24), to display reported relative abundance-distributions beyond the scope of the sampled locations. Species with severely isolated relative abundance-distribution profiles (e.g. leopard, Panthera pardus, Cape clawless otter, Aonyx capensis and honey badger, Mellivora capensis), species with low overall incidence (e.g. aardwolf, Proteles cristata and Smith's red rock rabbit, Pronolagus rupestris), and non-native species with high incidences (e.g. feral dog, Canis familiaris and feral pig, Sus scrofa) were identified, and can now be prioritised further for future research and conservation efforts within the BMC region.