In this paper, we present the lectotypification of 57 names of ferns and lycophytes for a total of 60 taxa studied. These names belong to species that occur mainly in the Guianas and other localities in the Neotropical region. Most of these names (46) are synonyms and others (14) are names in use. Among these 57 lectotypifications, 45 are here designated, 10 of them were already made by previous authors, but were overlooked by subsequent authors or for which we give additional information, and two others are second-step lectotypifications (Adiantum tomentosum and Pteris biformis). Two holotypes have been confirmed, and for one name (Adiantum petiolatum), typification was not possible to make because we did not find the original material. For all cited types, we provide information about the presence of barcode or accession numbers. This information allows easy localization of the type specimens and their image through the Word Wide Web.