Gladiolus oreophilus Eker & Sağıroğlu sp. nova (Amaryllidaceae) is described as a new species from the Hatay Province, Turkey. It resembles G. kotschyanus, G. alanyensis and G. adanus, but it clearly differs from these three species by capsule size, flower colour, number and width of leaves, seed shape and ornamentation, and shapes of periclinal cell walls and testa epidermal cells. Herein, we provide the morphological features, distribution, habitat characteristics, conservation assessment, and micromorphological characteristics of seeds of G. oreophilus.