Basiria iranicasp. nov., recovered from the rhizospheric soil of pomegranate in Khuzestan province, southwestern Iran, is described and illustrated based upon morphological and molecular characters. The new species is characterized by its lateral fields composed of four incisures, with non areolated bands, lip region smooth and continuous with body contour, stylet 11–13 µm long, basal bulb pyriform to slightly cylindrical, V = 62 (60.3–63.3), tail elongate-conoid, tail tip pointed to filiform and males with 20 (19–21) µm long spicules. Morphologically, the new species comes close to B. berylla, B. duplexa, B. elegans, B. graminophila, B. hissariensis, B. khouzestanensis and B. paraobliqua. Morphological comparisons with these species are discussed. The sequence of the D2–D3 expansion segments of 28S rDNA gene for the new species was also used for molecular phylogenetic analysis. The phylogenetic relationships of the new species in relation to representatives of the family Tylenchidae, obtained from Bayesian inference (BI) analysis of the D2–D3 sequences, are presented and discussed. The new species clusters in a subclade containing B. aberrans, three unidentified species of the genus Basiria and B. khouzestanensis with high support.