Small noncoding RNAs deep sequencing (sncRNA-Seq) has become a routine for sncRNA detection and quantification. However, the software packages currently available for sncRNA annotation can neither recognize sncRNA variants in the sequencing reads, nor annotate all known sncRNA simultaneously. Here, we report a novel anchor alignment-based small RNA annotation (AASRA) software package ( https://github.com/biogramming/AASRA). AASRA represents an all-in-one sncRNA annotation pipeline, which allows for high-speed, simultaneous annotation of all known sncRNA species with the capability to distinguish mature from precursor microRNAs, and to identify novel sncRNA variants in the sncRNA-Seq sequencing reads.
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31 March 2021
AASRA: an anchor alignment-based small RNA annotation pipeline
Chong Tang,
Yeming Xie,
Mei Guo,
Wei Yan
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Biology of Reproduction
Vol. 105 • No. 1
July 2021
Vol. 105 • No. 1
July 2021
bioinformatics
precursor microRNA
RNA-seq
sequence alignment
small RNA annotation
