Several leukocyte populations have been described within the pregnant mouse uterus, some of which express the integrin beta 7 (ITGB7). Here we demonstrate that the majority of the ITGB7 decidual leukocytes belong to the dendritic cell (DC) lineage. By multiparameter flow cytometric analysis we demonstrated the existence of three distinct DC subsets, characterized by differential expression of ITGA4/ITGB7 (formerly alpha4beta7-integrin) and ITGAE/ITGB7 (formerly alphaEbeta7-integrin). Importantly, the predominant DC subsets reside in distinct microdomains of the Day 9 pregnant mouse uterus. ITGAX ITGAM^med ITGA4/ITGB7 ITGAE⁻ (formerly CD11c CD11b^med alpha4beta7 alphaE⁻) cells represent the majority of DCs in the vascular zone (VZ), whereas ITGAX ITGAM⁻ ITGAE/ITGB7 (formerly CD11c CD11b⁻ alphaEbeta7 ) DCs are mainly located in the lower central decidua basalis (cDB) and the underlying myometrium. A population of ITGAX ITGAM^low DCs lacking ITGB7 are restricted to the cDB. Confocal microscopy studies show direct contact of VZ DCs with uterine natural killer (uNK) cells, suggesting a functional relationship between both cell populations. Collectively, our data identify three phenotypically distinct DC subsets residing in distinct microdomains of the uterus. The differential expression of ITGA4/ITGB7 and ITGAE/ITGB7 suggests distinct functional roles of the different DC subsets during early pregnancy.