White-footed mice (Peromyscus leucopus) are important host reservoirs for Borrelia burgdorferi and Rickettsia rickettsii. These bacteria are responsible for the tick-borne illnesses Lyme disease and Rocky Mountain spotted fever, respectively. These illnesses infect Ohio citizens annually, as well others in the larger range where these ticks are found. The goal of this study was to test white-footed mice and ticks for B. burgdorferi and R. rickettsii at Capital University's Primmer Outdoor Learning Center located in Logan, Ohio during the summer of 2020. Blood samples and ticks were collected from mice (n = 16) over the span of six live-trapping sessions in a woodlot and fencerow on the property. Ticks were also collected from four other locations using the drag-cloth method. DNA was extracted from the blood samples (n = 13) and ticks (n = 16) and tested for the presence of B. burgdorferi and Rickettsia sp. using conventional PCR and agarose gel electrophoresis. Relative abundance of mice was slightly higher in the fencerow than in the woodlot. Only American dog ticks (Dermacentor variabilis) were found in this study. B. burgdorferi was not detected, while Rickettsia sp. were confirmed for one mouse and 14 ticks. The results from this study increase our understanding of tick-borne pathogens within the state of Ohio and suggest we need to take steps to minimize risk of disease exposure at the University field site. In the future, this work can be used to inform models to simulate tick-borne pathogen dynamics in southeastern Ohio for both Lyme disease and Rocky Mountain spotted fever.