Kułaj, D., Pokorska, J., Ormian, M. and Dusza, M. 2015. Short Communication: New alleles at the BoLA-DQA1 locus in Holstein Fresian cattle. Can. J. Anim. Sci. 95: 161-164. The aim of the study was to analyze genetic polymorphism at locus BoLA-DQA1 in Polish Holstein-Friesian cattle, using next generation sequencing technology (Miseq sequencer - Illumina). In the studied cattle population we identified 14 alleles, of which three were determined for the first time (BoLA-DQA1*0106, BoLA-DQA1*3201, BoLA-DQA1*3301). The frequencies of these alleles were respectively: 0.0423, 0.0282, 0.0282. At locus BoLA-DQA1 the highest frequency was found for the BoLA-DQA1*10011 and BoLA-DQA1*0101 alleles and their frequencies were respectively: 0.3592 and 0.2606. The frequencies of other alleles identified in the studied population ranged from 0.0070 to 0.0704. The results obtained in this study indicate that the BoLA-DQA1 locus is highly polymorphic and new alleles are still identifiable.