Microplate assay for boron analysis in soil and plant tissue

Kebba Sabally; Joann K. Whalen; Bao-Luo Ma

doi:10.1139/cjss-2016-0125

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Kebba Sabally,¹ Joann K. Whalen,^2,* Bao-Luo Ma³

¹K. Sabally. School of Dietetics and Human Nutrition, Macdonald Campus of McGill University, Ste-Anne
²J. K. Whalen. Department of Natural Resource Sciences, Macdonald Campus of McGill University, Ste-An
³B.-L. Ma. Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, ON K1A 0

^*Corresponding author: J.K. Whalen (email: joann.whalen@mcgill.ca).

Abstract

The boron concentration in soil extracts and ashed plant tissue was quantified with a rapid and reproducible microplate assay. The microsized azomethine-H method required adjustment to reduce pH and chemical interferences in soil and plant tissue samples. Microplate spectrophotometry permits replication and quality control and is suitable for high-throughput analysis.

Citation Download Citation

Kebba Sabally, Joann K. Whalen, and Bao-Luo Ma "Microplate assay for boron analysis in soil and plant tissue," Canadian Journal of Soil Science 97(2), 334-338, (5 April 2017). https://doi.org/10.1139/cjss-2016-0125

Received: 9 October 2016; Accepted: 1 April 2017; Published: 5 April 2017