The objective of this study was to survey the ants in an area of natural regeneration in the municipality of Alta Floresta, Mato Grosso, Brazil. Sampling of the myrmecofauna was performed by means of soil traps containing preservative liquid, distributed along a transect. After 48 hours in the field, the specimens were collected, quantified and separated on the basis of morphospecies classification, and sent for taxonomic identification. A total of 586 individuals were sampled in five genera and three subfamilies (Myrmicinae, Dolichoderinae and Formicinae), with the most abundant species being Dorymyrmex brunneum and Pheidole obscurithorax.