Spider diversity is an important pillar of biological control in agriculture, which has attracted the attention of ecologists globally. Despite its economic and ecological importance in agriculture, limited work is available from Turkey regarding spider diversity. This study investigated spider fauna of some major (wheat, maize and vegetables) and minor (sunflower, groundnut, tobacco, sesame and potato) field crops in southeastern Anatolia region, Turkey. Spider samples were collected from 16 different localities (115 fields) with sweep net and D-Vac machine. A total 212 specimens were classified as 41 species and 21 genera belonging to 18 families. The most commonly observed families were Philodromidae, Salticidae and Thomisidae. The highest spider diversity was found in cereal crops (23 species) followed by vegetable crops (22 species), while sunflower, lentil and potato (2 species each) had the lowest diversity. Thanatus sp., Evarcha sp., Phlegra sp. were the most abundant species in wheat. Cyclosa algerica Simon, 1885 was the first record in spider fauna from maize fields in Turkey. The current study improves the knowledge on spider diversity in Turkey, which can be used in future studies.