This study examines the efficacy of various artificial diets on the mortality rates of Bemisia tabaci (whiteflies) and evaluates the insecticidal properties of protein extracts from selected tropical plants. Colonies of B. tabaci were maintained in a controlled greenhouse environment and fed with different artificial diets. A bioassay method using vials with two hollow side arms was employed for administering the diets and for infestation with whiteflies. The biotype of B. tabaci was determined using a RAPD PCR test. Three distinct artificial diets were tested, alongside periodic renewals from natural hosts to maintain genetic vigor in the laboratory colony. Bioassays were conducted to assess the effectiveness of these diets and to determine the insecticidal activity of protein extracts from seven tropical plants. The results indicated that while the complex diet without TRIS led to 100% mortality within six days, other diets showed variable mortality rates with no significant difference in mean mortality. Our study found high mortality rates when CHAPS, a common solubilizing agent for membrane proteins, was used in the extraction process, suggesting its toxicity to B. tabaci. Among the tested plant protein extracts, four exhibited over 90% mortality at 72 hours, indicating potential as natural insecticides. The research highlights the need for careful selection of solvents in bioassays and points to the potential of young leaves from tropical plants as sources of anti-whitefly compounds.