Some terrestrial isopods, as many other organisms, were able to colonize several continents across the world due to human activities. Nonetheless, many tropical or subtropical species, that are found in Europe, seem to be restricted to man-made infrastructures, such as greenhouses. This is the case with members of the family Styloniscidae, Styloniscus mauritiensis and S. spinosus. For the first time, we recorded these two species in the Iberian Peninsula and updated the distribution of the family Styloniscidae in the region. Styloniscus mauritiensis was recorded in greenhouses in the Madrid province, Spain, while both S. mauritiensis and S. spinosus were found in the Oporto region, Portugal, in outdoor environments. These represent the first records of these species in Europe outside man-made infrastructures. Finally, we provided detailed information to facilitate the easier identification of the three Styloniscidae species now known to occur in the Iberian Peninsula and several other parts of the world.