The 2023 checklist for Pennsylvania bryophytes lists 547 taxa including 426 mosses, 118 liverworts, and 3 hornworts, but only 48 confirmed moss species and one liverwort species collected during the last 100 years from Perry County. As a result of fieldwork reported herein and review of herbarium data from 1923 to present, an additional 49 moss and 2 liverwort species are now included in the Perry County bryoflora. Significant bryophyte species reported from the county include the moss Bucklandiella venusta, which represents the first collection of this species in Pennsylvania during the past 100 years. Currently, 97 moss and 3 hepatic taxa are reported from Perry County, Pennsylvania.