The sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), which attacks sugarcane, corn, sorghum, sudan grass and rice, is a major agronomic pest in the southeastern United States. This study was conducted to investigate the efficacy of different rates of Dermacor-X-100® (active ingredient, chlorantraniliprole) seed treatments on D. saccharalis in rice in the laboratory, greenhouse and field. In laboratory assays using cut stems, Dermacor-X-100® resulted in 40–50% more mortality than in the control, while in greenhouse intact plant assays Dermacor-X-100® resulted in approximately 70–80% higher mortality compared to controls. In the field study, Dermacor-X-100® seed treatment resulted in significantly lower numbers of borer entry and exit holes per plant. Results from this study suggest that Dermacor-X-100® seed treatment could be used as a valuable component of integrated pest management program for stem borers in Louisiana rice. As the boring behavior of larvae, life cycle and injury caused by most stem borers are generally similar, and because the active ingredient in Dermacor-X-100® is highly efficacious against lepidopteran pests, results for D. saccharalis may extend to management of other stem boring species as well.