The Asiatic cheetah (Acinonyx jubatus venaticus), has disappeared in major parts of its mountainous habitats in west Asia. Asiatic cheetah is a critically endangered subspecies currently found only in central Iran. In this study, the feeding habits of Asiatic cheetah was investigated in two reserves in central Iran. Diet and prey selection of cheetah were assessed using two biomass models along with Jacob's selectivity index. We found no significant difference between biomass consumption based on each model between the reserves or between models inside each reserve. Over 90 % of species diet was composed of three medium-sized ungulates (i.e. 15–40 kg): wild sheep, wild goat and chinkara. Hares and other small mammals (less than 15 kg) made up the remainder of their diet. Asiatic cheetahs mainly preyed on wild sheep (Ovis vignei) which composed more than 50 % of consumed biomass. Wild sheep was identified as single staple prey, while chinkara (Gazella bennettii) was the most preferred prey despite its lower occurrence. Our study indicates that wild sheep, wild goat and especially chinkara are critical for persistence of Asiatic cheetah and must be particularly considered in conservation plans.