We describe a new species of Stefania from the summit of Cerro Autana in Amazonas, Venezuela. It is the westernmost species hitherto known for the genus, being 200 km northwest of Cerro Huachamacari, the nearest known locality for any other Stefania. The new species is distinguished from other species of Stefania by the following combination of characters: fronto-parietal ridges present but reduced, foot webbing basal, discs on fingers and toes small, post-tympanic warts absent, and head as long as wide. Based on these traits, the species can be placed in the Stefania evansi group of Rivero.