Somatic embryogenesis was induced from suspension cultures (derived from leaf callus) of an important medicinal plant, Plumbago rosea L. While acetylsalicylic acid (ASA) alone induced embryogenesis, indole-3-acetic acid (IAA) failed to elicit a similar response. This is the first time that ASA-induced somatic embryogenesis has been reported in cultured cells. Optimal embryogenic response per culture was observed in Murashige and Skoog's medium containing a combination of ASA (8.32 μM) and IAA (5.06 μM), but 1-naphthaleneacetic acid and indole-3-butyric acid individually did not induce somatic embryogenesis. Increase in the concentration of ammonium enhanced the number of embryos formed per culture. Accumulation of plumbagin, an important naphthoquinone and a medicinal compound, was three times higher in embryogenic compared to non-embryogenic suspensions.
How to translate text using browser tools
1 March 2004
ACETYLSALICYLIC ACID AND AMMONIUM-INDUCED SOMATIC EMBRYOGENESIS AND ENHANCED PLUMBAGIN PRODUCTION IN SUSPENSION CULTURES OF PLUMBAGO ROSEA L.
P. KOMARAIAH,
G. JOGESWAR,
S. V. RAMAKRISHNA,
P. B. KAVI KISHOR
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
In Vitro Cellular and Developmental Biology - Plant
Vol. 40 • No. 2
March 2004
Vol. 40 • No. 2
March 2004
acetylsalicylic acid
cell suspensions
plumbagin
Plumbago rosea
somatic embryogenesis