Capsicum regeneration is often obtained through direct (adventitious) regeneration and only a few reports claim indirect regeneration (through callus) as an option to obtain complete plants. A possible reason for this may be because Capsicum cultures offer a narrow window in time for the regeneration process to occur, and after that, the ability to regenerate plants rapidly diminishes. In this study, the C. baccatum radicle-side half-seed was the explant of choice to induce the formation of callus on semisolid Murashige and Skoog (1962) medium supplemented with 5 mg l−1 (22.2 μM) 6-benzylaminopurine, 1 mg l−1 (5.7 μM) indole-3-acetic acid, and 2 mg l−1 (6.1 μM) gibberellic acid. Organogenic calluses developed within 4–5 mo. and were subcultured every 2 mo. thereafter. Eventual bud elongation occurred and these shoots developed into complete plants after transfer to medium without plant growth regulators. The organogenic callus type retained its organogenic ability for more than 3 yr.
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1 July 2005
LONG-LASTING CAPSICUM BACCATUM ‘ORGANOGENIC CALLUS’ FORMATION
LUIS L. VALERA-MONTERO,
GREGORY C. PHILLIPS
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In Vitro Cellular and Developmental Biology - Plant
Vol. 41 • No. 4
July 2005
Vol. 41 • No. 4
July 2005
chile pepper
half-seed explant
in vitro
Plant regeneration
shoot organogenesis