Rice stripe disease, which is caused by Rice stripe virus (RSV), is one of the most serious viral diseases of rice. RSV is transmitted in a persistent manner by Laodelphax striatellus (Fallén). The incidence of the disease can be estimated from the density of viruliferous vectors. Understanding seasonal changes of the percentage of viruliferous L. striatellus can facilitate forecasting and controlling the disease. In paddies, the percentage of viruliferous insects fluctuated in phase with the rate of detection of RSV-infected rice; it gradually increased from July to August, plateaued or temporarily declined in September, and increased sharply on ratoons in October. These findings indicate that horizontal transmission of RSV from diseased plants to vector insects occurred frequently, and the insects acquired RSV from the ratoons. However, the percentages of viruliferous insects overwintering in poaceous weeds, the main hosts for L. striatellus in winter, were lower than those in ratoons. Few L. striatellus that acquired RSV from ratoons seemed to move to overwintering sites and transmit the virus to the next generation. However, there was a tendency for the percentages of viruliferous overwintering insects to be higher on paddy ridges than in river levees. Insects could probably move from ratoons to poaceous weeds when the weeds were near a paddy. Although increasing percentage of viruliferous insects on ratoons seem to have relatively little impact on RSV dynamics in the next crop season, appropriate weed management around paddies is still needed to reduce the incidence of rice stripe disease.