The melon fruit fly, Bactrocera cucurbitae (Coquillett) and the pumpkin fruit fly, Bactrocera tau (Walker) (Diptera: Tephritidae), are important invasive pests on Cucurbitaceous hosts. The acute toxicity of five insecticides to females of these two fruit fly species was bio-assayed by membrane method, and effects of their sublethal concentration on the survival, reproduction, and ovary development of females were evaluated in the laboratory. Results showed that based on the LC₅₀ values, both B. cucurbitae and B. tau were the most susceptible to trichlorfon. After treatment with sublethal concentration (LC₃₀) of trichlorfon, abamectin+β-cypermethrin, spinetoram, and lambda-cyhalothrin, the female survival days of the two Bactrocera species were significantly shortened compared with the control. Moreover, the fecundity of two Bactrocera species was remarkably decreased, after exposure to abamectin+β-cypermethrin and trichlorfon LC₃₀. However, the sublethal concentration (LC₃₀) of insecticides had no significant influence on the egg hatchability of the fruit flies. Furthermore, after treatment with abamectin+β-cypermethrin LC₃₀, the ovary length, width, and egg load of B. cucurbitae were significantly lower than that of the control; however, only the ovarian length and egg load of B. tau were significantly decreased on the 16th and 20th day. In conclusion, abamectin+β-cypermethrin has an excellent insecticidal activity against B. cucurbitae and B. tau.