The Asian corn borer, Ostrinia furnacalis (Guenée), is a notorious pest of maize that migrates seasonally in Asia. Two migration peaks were found on Beihuang island in the Bohai Strait of China by observing the number of migrants. However, the origins and host plants of the migrants in the two migration periods remain unclear. Here, stable hydrogen (δ²H) and carbon (δ¹³C) isotope levels were measured to infer the origin and host plants of the O. furnacalis captured on Beihuang island in 2017–2019. δ²H in wings of spring-summer O. furnacalis captured from May to June ranged from –99 to –56‰, while that of autumn migrants from August to September ranged from –127 to –81‰. Based on the linear relationship between δ²H in the wing of migrants (δ²H_w) and δ²H in precipitation (δ²H_p), the spring-summer O. furnacalis likely originated from the summer maize area in the Huang-Huai-Hai Plain in China. In contrast, the autumn migrants came from the northern spring maize area in Liaoning, Jilin and Inner Mongolia. Based on δ¹³C, the spring-summer migrants fed on both C₃ plants such as wheat (47.76%) and C₄ weeds or belonged to the over winter individuals in maize field (52.24%), while the autumn migrants mainly fed on maize (C₄, 91.21%). The results point to a northward migration in spring-summer and southward migration in autumn of O. furnacalis. Our study gives an important knowledge for improving the forecasting and management level of this pest.