In ART, once an ovum is successfully yielded, embryologists have to progress the treatments as arranged regardless of the quality of semen. Sperm qualities are, therefore, the most variable factor in each case. Human ejaculate contains heterogeneous sperm populations, which possess a variety of abnormalities at nuclear, cytoskeletal and organelle levels. Embryologists are certainly well informed about physiology and genetics of ova and embryos, moreover, it is also essential for them to study how to evaluate and prepare the sperm according to their physiology.