Environmental and Geo-spatial factors have long been considered as crucial determinants of species composition and distributions. However, quantifying the relative contributions of these factors for the alpine ecosystems is lacking. The Tibetan Plateau has a unique ecological environment and vegetation types. Our objectives are to quantify the spatial distributions of plant communities on the Northern Tibetan Alpine grasslands and to explore the relationships between vegetation composition, Geo-spatial factors and environmental factors. We established 63 field plots along a 1200-km gradient on the Northern Tibetan Plateau Alpine Grassland and employed the two-way indicator species analysis (TWINSPAN) and the detrended canonical correspondence analysis (DCCA). Fourteen communities of alpine grassland were identifiable along the transect and consisted of three vegetation types: Alpine meadow, Alpine steppe, and desert steppe. Vegetation composition and spatial distribution appeared to be largely determined by mean annual precipitation and less influenced by temperature. A large fraction (73.5%) of the variation in vegetation distribution was explained by environmental variables along this transect, somewhat less by Geo-spatial factors (56.3%). The environmental and Geo-spatial factors explained 29.6% and 12.3% of the total variation, respectively, while their interaction explained 43.9%. Our findings provide strong empirical evidence for explaining biological and environmental synergetic relationships in Northern Tibet.