Aedes triseriatus is the principal vector of La Crosse virus (LACv), which is the most common cause of pediatric arboviral encephalitis in North America. Here we report a novel species-specific polymerase chain reaction (PCR) assay that differentially identifies Ae. triseriatus and Ae. hendersoni. Because these 2 sibling species differ in their abilities to transmit LACv, accurate identification is critical for surveillance, research, and control programs. This duplex assay can detect the presence of both species in a single PCR reaction and is therefore simpler and faster than previously reported methods.
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1 June 2014
Molecular Identification of Aedes triseriatus and Aedes hendersoni by a Novel Duplex Polymerase Chain Reaction Assay
Robert Wilson,
Robin Harrison,
Michael Riles,
Gideon Wasserberg,
Brian D. Byrd
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Aedes hendersoni
Aedes triseriatus
La Crosse encephalitis
Ochlerotatus hendersoni
Ochlerotatus triseriatus