Fish community studies were carried out in the Little River basin of western Kentucky in 2000 and 2003. Thirteen families and 45 species of fish were collected at 16 sites. Thirty-three percent of all taxa were considered intolerant of high turbidity, siltation, and low dissolved oxygen. The most frequently encountered species were scarlet shiner (15 of 16 sites), striped shiner (14 of 16 sites), bluntnose minnow (13 of 16 sites), and longear sunfish at 13 of 16 sites. Other taxa occurring at 75% of all sites included bluegill, largescale stoneroller, banded sculpin, and green sunfish. The highest number of species (21) was found at Site 11 on the Sinking Fork. No federally threatened or endangered species were found. The Little River continues to be an impaired system for aquatic biota.