Yersinia enterocolitica serotype 0:5,27 biotype 2 was isolated from the intestinal contents of a common garter snake (Thamnophis sirtalis). The isolate possessed virulence associated phenotypes in all tests conducted. It was susceptible to amikacin, ampicillin/sulbactam, aztreonam, cefoperazone, cefotaxime, ceftazidime, ceftriaxone, cefuroxime, ciprofloxacin, gentamicin, imipenem, mezlocillin, norfloxacin, piperacillin, ticarcillin/clavulanic acid, trimethoprim-sulfamethoxazole, tobramycin, chloramphenicol and tetracycline. The isolate harbored the virulence plasmid.