Muskrat (Ondatra zibethicus) immunoglobulin fraction was separated from whole serum by Protein A Sepharose chromatography. In serum electrophoresis, this fraction had a gamma motility; when electrophoresed on a polyacrylamide gel with sodium dodecyl sulfate it resolved into two protein bands of approximately 52 and 25 kilodaltons, respectively These bands were consistent with molecular weights of known heavy and light chains of immunoglobulin G (IgG) in other closely related species. Furthermore, the putative muskrat immunoglobulins had a strong cross-reactivity with mouse IgG1, IgG3, and kappa chain in an enzyme-linked immunosorbent assay. We propose, that the proteins bound to the Protein A Sepharose represent muskrat immunoglobulins of the IgG class.
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1 October 1996
Brief Characterization of Muskrat (Ondatra zibethicus) Immunoglobulin G (IgG) Separated from Serum on Protein A
Joanna D. Borucinska,
Ihsan Ayoub,
Antonio E. Garmendia
Journal of Wildlife Diseases
Vol. 32 • No. 4
October 1996
Vol. 32 • No. 4
October 1996
IgG separation
muskrat
Ondatra zibethicus