A buffalo (Syncerus caffer) was inoculated intravenously and intradermally with a high dose of Allerton-type herpes virus isolated from a sick buffalo during an outbreak of a disease in buffaloes in Serengeti National Park, Tanzania, in 1969. The animal reacted severely and generalized skin nodules as well as oral and nasal lesions were observed. Viraemia was detected and the virus was isolated from swabs and also from ticks which were fed on the animal during the viraemic phase. The buffalo produced neutralizing antibody and recovered after a long illness. It would therefore appear that under certain conditions the Allerton-type herpes virus can be of high pathogenicity for buffaloes.