A national survey questionnaire was conducted in 32 zoos throughout 17 U.S. states between February and May 2008. The questionnaire consisted of six questions that evaluated rabies prevalence on zoo grounds; captive mammalian bites among zoo visitors and personnel; and the outcome of each incident. The survey was completed by zoo staff after review of their most recent bite incident reports. Rabies was documented, albeit minimally, on zoo grounds in both wild and collection animals. The information collected documented that mammalian bites occur commonly. An average of 9.1 bite incidents per zoo involving zoo visitors were reported in the last 5 yr, compared to an average of 7.5 bite incidents per zoo involving personnel within the last 5 yr. Zoo personnel had a larger variety of mammals inflicting bites of greater injury severity, with an average rating 3.83 out of 5. While victim profile and severity of the wound differed between visitor and personnel bites, the majority of bites were not reported to the local health department. Lack of reporting may be due to low rabies risk, fear of media involvement, and an unknown conclusion for the offending animal. Animals involved in reported attacks had an average quarantine of 47.5 days when the bite involved a zoo visitor versus an average quarantine of 18 days when the bite involved personnel. These results demonstrate the need for a standardized protocol following a bite incident, including cooperation with the local health department, as necessary.