A retrospective study of neoplasia was conducted from necropsy and histologic reports of 446 cranes representing all 15 extant species. Cases were obtained from the International Crane Foundation (ICF), Northwest ZooPath (NWZP), and six other zoologic institutions in the United States during 1993 to 2019. Only reports from ICF (n = 61) and NWZP (n = 374) were used for estimates of disease prevalence. Overall prevalence of neoplasia was 7.35% (32 of 435), with a metastasis rate of 31.8%. Seventeen types of neoplasms were identified. Geriatric cranes were the most common age class affected (60%). The digestive system was most frequently involved (n = 12; 27.3%), followed by urinary (n = 8; 18.2%) and integumentary systems (n = 6; 13.6%). Carcinoma was the most common tumor type across all species (n = 15; 34.0%) followed by benign epithelial tumors (adenomas; n = 11; 25.0%). Multiple neoplasms were observed in only one crane. Of the cases with metastasis, hematopoietic, reproductive, and respiratory tumors had 100% metastasis (2 of 2, 2 of 2, and 3 of 3, respectively), whereas tumors of the integumentary, nervous, and musculoskeletal systems had no evidence of metastasis (0 of 7, 0 of 3, and 0 of 1, respectively). Overall, Gruidae species were found to have a high prevalence of neoplasia but a low rate of metastasis compared with other avian species with the highest species-specific rates in Eurasian (Grus grus), demoiselle (Anthropoides virgo), and wattled cranes (Bugeranus carunculatus) (40.0%, 21.4%, and 19.0%, respectively). This is the first large-scale study of neoplasia in cranes.