Praying mantises have gained increasing popularity as pets and may be anesthetized to allow morphological identification and clinical procedures. The aim of this study was to evaluate the effects and anesthetic-related complications of isoflurane in oxygen in 15 client-owned budwing mantises (Parasphendale agrionina) undergoing photographic identification. Each mantis was placed in a self-constructed anesthetic chamber and exposed to a mixture of approximately 5% isoflurane in oxygen until anesthetic induction was achieved. Time to anesthetic induction, time to recovery from anesthesia, and quality of anesthesia based on scoring of righting reflex, front legs withdrawal, and muscular tone, were evaluated and recorded, as well as the occurrence of undesired effects. In most mantises, immobility, loss of righting reflex, and myorelaxation were achieved within minutes after the beginning of isoflurane exposure and lasted a time sufficient to allow completion of the procedure. However, mantises aged ≥12 mo had longer time to anesthetic induction (4.3 ± 0.6 m), as well as shorter time to recovery (19.0 ± 8.0 m), than mantises aged <12 mo (0.8 ± 0.4 and 30.0 ± 15.0; P < 0.05). Complications were not observed, and all mantises were returned to their owner on the same evening.