Little is known about composition of bat communities and their acoustic activity in the northern boreal forests. Acoustic and capture surveys were conducted in the Mecatina River Ecoregion (MRE) in south central Labrador. We acoustically surveyed forest edges, streams, and ponds and netted and trapped bats near streams and along forest edges. The acoustic survey showed greater bat activity at streams and ponds. The capture survey confirmed the presence of Myotis lucifugus (Little Brown Bat) and M. septentrionalis (Northern Long-eared Bat) in the MRE, the latter showing signs of reproduction. Together these results support the idea that riparian areas in the boreal forest are important landscape features for bats in the genus Myotis.