Myotis lucifugus (Little Brown Myotis) is a regionally migrating bat, widely distributed throughout North America. Through long-term monitoring systems deployed in 2 locations in Newfoundland, Canada, we recorded the movement of an adult female of at least 375 km over a period of 4 nights. Although not the longest travel distance recorded for this species, this observation emphasizes the capabilities of Little Brown Myotis for long-distance flights, the potential connectivity among distant maternity groups, and the value of long-term monitoring programs. To fully understand the movement patterns of Little Brown Myotis, advancements in technology and greater research effort are needed.