Imaging of Photodynamically Generated Singlet Oxygen Luminescence In Vivo¶

Mark J. Niedre; Michael S. Patterson; Anoja Giles; Brian C. Wilson

doi:10.1562/2005-03-15-TSN-462R.1

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Mark J. Niedre,¹ Michael S. Patterson,² Anoja Giles,¹ Brian C. Wilson^1,*

¹aDepartment of Medical Biophysics, Ontario Cancer Institute and University of Toronto, Toronto, Onta
²bJuravinski Cancer Center and McMaster University, Hamilton, Ontario, Canada

^**To whom correspondence should be addressed: Division of Biophysics and Bioimaging, Ontario Cancer Institute, 610 University Avenue, Toronto, ON M5G 2M9, Canada. Fax: 416-946-6529; e-mail: wilson@uhnres.utoronto.ca

Abstract

We describe a novel scanning-laser system for imaging type-II photodynamically generated singlet oxygen (¹O₂[¹Δ_g]) luminescence and demonstrate it in vivo in an intradermal tumor model in mice. We verify the strong oxygen-dependence of the signal and show that the images are near the practical resolution limit.

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Mark J. Niedre, Michael S. Patterson, Anoja Giles, and Brian C. Wilson "Imaging of Photodynamically Generated Singlet Oxygen Luminescence In Vivo," Photochemistry and Photobiology 81(4), 941-943, (1 July 2005). https://doi.org/10.1562/2005-03-15-TSN-462R.1

Received: 4 March 2005; Accepted: 1 April 2005; Published: 1 July 2005

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